Cloning, molecular evolution and functional characterization of ZmbHLH16, the maize ortholog of OsTIP2 (OsbHLH142)
نویسندگان
چکیده
The transcription factor ZmbHLH16, the maize ortholog of OsTIP2 (OsbHLH142), was isolated in the present study. Tissue expression analysis showed that ZmbHLH16 is preferentially expressed in male reproductive organs. Subcellular location analysis of ZmbHLH16 via rice protoplast indicated that it is located in the nucleus. Through nucleotide variation analysis, 36 polymorphic sites in ZmbHLH16, including 23 single nucleotide polymorphisms and 13 InDels, were detected among 78 maize inbred lines. Neutrality tests and linkage disequilibrium analysis showed that ZmbHLH16 experienced no significant evolutionary pressure. Yeast one-hybrid experiment showed that the first 80 residues in the N-terminus of ZmbHLH16 had transactivation activity, whereas the full length did not. Genome-wide coexpression analysis showed that 395 genes were coexpressed with ZmbHLH16. Among these genes, the transcription factor ZmbHLH51 had similar expression pattern and identical subcellular localization to those of ZmbHLH16. Subsequently, the interaction between ZmbHLH51 and ZmbHLH16 was verified by yeast two-hybrid experiment. Through yeast two-hybrid analysis of series truncated ZmbHLH16 fragments, we found not only the typical bHLH domain [175-221 amino acids (a.a.)], but also that the 81-160 a.a. and 241-365 a.a. of ZmbHLH16 could interact with ZmbHLH51. All these results lay the foundation for further understanding the functions of ZmbHLH16.
منابع مشابه
Cloning , molecular evolution and functional characterization of 1 ZmbHLH 16 , the maize ortholog of OsTIP 2 ( OsbHLH 142 )
Basic helix-loop-helix (bHLH) transcription factors play key roles in plant male 10 reproduction. More than 14 bHLH proteins related to pollen development have been cloned from rice 11 and Arabidopsis. However, little is known about the role of the bHLH family in maize microspore 12 development. In this study, the bHLH transcription factor ZmbHLH16 was cloned. ZmbHLH16 13 shares high similarity...
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عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2017